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RNase H1-Dependent ASO Cleavage

RNase H1-Dependent ASO Cleavage

A gapmer antisense oligonucleotide binds a complementary RNA sequence and recruits RNase H1 to cleave the RNA strand of the hybrid.

Overview

EXPLORE BY LEVEL

A gapmer is a designed strand that pairs with an RNA message. The paired region can recruit RNase H1, an that cuts the RNA, so the message can be degraded.

Quick Facts

AT A GLANCE
Explore nextRNA therapeutics

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See how RNase H1-Dependent ASO Cleavage links to other biological records.

Open network
TherapyAntisense Oligonucleotidescan useProteinRNase H1recruitsMoleculeMessenger RNA (mRNA)can cause cleavage of
All 3 Structured Connections
Antisense Oligonucleotides · TherapyCAN USE →RNase H1-Dependent ASO Cleavage · Process
RNase H1-Dependent ASO Cleavage · ProcessRECRUITS →RNase H1 · Protein
RNase H1-Dependent ASO Cleavage · ProcessCAN CAUSE CLEAVAGE OF →Messenger RNA (mRNA) · Molecule

Recommended Reading

Trusted references for learning more about RNase H1-Dependent ASO Cleavage.

articleRNA cleavage products generated by antisense oligonucleotides and siRNAs are processed by the RNA surveillance machineryNucleic Acids Research · PMID 26843429 reviewMechanisms and strategies for effective delivery of antisense and siRNA oligonucleotidesPubMed Central
Research Claims

Gapmer activity depends on oligonucleotide gap and wing chemistry, affinity, RNase H1 recruitment, transcript localization, sequence off-targets, and tissue exposure. Not all ASOs activate RNase H1.