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Protein

RNase H1

RNase H1

An enzyme that recognizes RNA–DNA hybrids and can cleave the RNA strand, a mechanism used by some antisense drugs.

Overview

EXPLORE BY LEVEL

When some antisense drugs pair with an RNA message, RNase H1 can recognize the hybrid and cut the RNA strand.

Quick Facts

AT A GLANCE
Explore nextRNA therapeutics

Follow the Connections

See how RNase H1 links to other biological records.

Open network
TherapyAntisense Oligonucleotidescan recruitProcessRNase H1-Dependent ASO Cleavagerecruits
All 2 Structured Connections
Antisense Oligonucleotides · TherapyCAN RECRUIT →RNase H1 · Protein
RNase H1-Dependent ASO Cleavage · ProcessRECRUITS →RNase H1 · Protein

Recommended Reading

Trusted references for learning more about RNase H1.

reviewMechanisms and strategies for effective delivery of antisense and siRNA oligonucleotidesPubMed Central reviewCurrent Challenges in Delivery and Cytosolic Translocation of Therapeutic RNAsPubMed Central articleRNA cleavage products generated by antisense oligonucleotides and siRNAs are processed by the RNA surveillance machineryNucleic Acids Research · PMID 26843429
Research Claims

RNase H1 activity depends on hybrid structure, gap length and chemistry, intracellular localization, and enzyme availability. Steric-blocking ASOs use a different mechanism and do not require RNA cleavage.